Uteroglobin/CC10 Polyclonal antibody
Uteroglobin/CC10 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 10490-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse lung tissue, human lung cancer tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse lung tissue |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 9 publications below |
| IF | See 9 publications below |
| IP | See 1 publications below |
| FC | See 3 publications below |
Product Information
10490-1-AP targets Uteroglobin/CC10 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Uteroglobin/CC10 fusion protein Ag0758 相同性解析による交差性が予測される生物種 |
| Full Name | secretoglobin, family 1A, member 1 (uteroglobin) |
| Calculated molecular weight | 10 kDa |
| GenBank accession number | BC004481 |
| Gene symbol | Uteroglobin |
| Gene ID (NCBI) | 7356 |
| RRID | AB_2183285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Uteroglobin/CC10 antibody 10490-1-AP | Download protocol |
| IF protocol for Uteroglobin/CC10 antibody 10490-1-AP | Download protocol |
| FC protocol for Uteroglobin/CC10 antibody 10490-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Lancet Respir Med Tropism, replication competence, and innate immune responses of the coronavirus SARS-CoV-2 in human respiratory tract and conjunctiva: an analysis in ex-vivo and in-vitro cultures. | ||
PLoS Pathog Lung adenocarcinoma originates from retrovirus infection of proliferating type 2 pneumocytes during pulmonary post-natal development or tissue repair. | ||
Am J Respir Cell Mol Biol DACH1 Attenuates Airway Inflammation in COPD by Activating NRF2 Signaling | ||
J Pathol Overexpression of the SARS-CoV-2 receptor ACE2 is induced by cigarette smoke in bronchial and alveolar epithelia. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tessa (Verified Customer) (02-04-2019) | Used in 20pcw human fetal lung and got nice staining. Looks very specific with low background. Found that it did not require antigen retrieval in cryosections. In fact it turned out better in sections where 10mM Na Citrate buffer at pH6 was not used.
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