Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCIF

WB analysis using 17711-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17711-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 17711-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 17711-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17711-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 17711-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17711-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of L02 using 17711-1-AP
IP result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.
IHC staining of mouse brain using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse pancreas using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CoraLite® Plus 488 E-cadherin antibody (CL488-20874, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of hTERT-RPE1 using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of MDCK using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).
IF Staining of NIH3T3 cells using 17711-1-AP
IF result of anti-ARL13B (17711-1-AP) in NIH3T3 cell by Dr. Sudipto.
IF Staining of hTERT-RPE1 using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).
IF Staining of hTERT-RPE1 using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).
IF Staining of MDCK using 17711-1-AP
Immunofluorescent images of MDCK cells stained with ARL13B rabbit pAb (17711-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure-Goat anti-Rabbit IgG(H+L) for 17711-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 66200-1-Ig.
IF Staining of MDCK using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of hTERT-RPE1 using 17711-1-AP
IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.
IF Staining of mouse embryonic fibroblasts using 17711-1-AP
IF result from Dr. Corbit, Kevin. anti-ARL13B (17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

Featured Product
KD/KO Validated

ARL13BA Polyclonal antibody

ARL13BA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17711-1-AP

Publications(869)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ARL13B, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (869)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	L02 cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue, rat kidney tissue
Positive IP detected in	L02 cells
Positive IHC detected in	mouse brain tissue, human liver tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	hTERT-RPE1 cells, NIH3T3 cells, MDCK cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 13 publications below
WB	See 99 publications below
IHC	See 38 publications below
IF	See 769 publications below
IP	See 3 publications below

Product Information

17711-1-AP targets ARL13BA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12015 Product name: Recombinant human ARL13B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC094725 Sequence: MFSLMASCCGWFKRWREPVRLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGKKIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGTSGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNEFGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGWGTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylation factor-like 13B
Calculated molecular weight	48 kDa
Observed molecular weight	40-48 kDa, 60 kDa
GenBank accession number	BC094725
Gene Symbol	ARL13B
Gene ID (NCBI)	200894
RRID	AB_2060867
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q3SXY8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARL13B, also named ARL2L1, is the Ras superfamily's small ciliary G protein. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B cause Joubert syndrome (JS), an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). This antibody detects two speciﬁc bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band will likely represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
IF protocol for ARL13BA antibody 17711-1-AP	Download protocol
IHC protocol for ARL13BA antibody 17711-1-AP	Download protocol
IP protocol for ARL13BA antibody 17711-1-AP	Download protocol
WB protocol for ARL13BA antibody 17711-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell The developmental basis of fingerprint pattern formation and variation Authors - James D Glover View Article
human	IF	Nat Biotechnol Guided self-organization and cortical plate formation in human brain organoids. Authors - Madeline A Lancaster View Article
mouse	IF	Nature Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche. Authors - David Willnow View Article
mouse	IF	Science Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium. Authors - Avishag Mytlis View Article
human	IF	Nature A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy. Authors - Adriana Sánchez-Danés View Article
mouse	IF	Nature Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Authors - Matthew B Johnson View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17711-1-AP

WB analysis of mouse liver using 17711-1-AP

WB analysis using 17711-1-AP

WB analysis of NIH/3T3 using 17711-1-AP

WB analysis of mouse kidney using 17711-1-AP

WB analysis of L02 using 17711-1-AP

WB analysis of mouse brain using 17711-1-AP

IP experiment of L02 using 17711-1-AP

IHC staining of mouse brain using 17711-1-AP

IHC staining of mouse brain using 17711-1-AP

IHC staining of human testis using 17711-1-AP

IHC staining of human testis using 17711-1-AP

IHC staining of human liver using 17711-1-AP

IHC staining of human liver using 17711-1-AP

IF Staining of mouse pancreas using 17711-1-AP

IF Staining of hTERT-RPE1 using 17711-1-AP

IF Staining of MDCK using 17711-1-AP

IF Staining of NIH3T3 cells using 17711-1-AP

IF Staining of hTERT-RPE1 using 17711-1-AP

IF Staining of hTERT-RPE1 using 17711-1-AP

IF Staining of MDCK using 17711-1-AP

IF Staining of MDCK using 17711-1-AP

IF Staining of hTERT-RPE1 using 17711-1-AP

IF Staining of mouse embryonic fibroblasts using 17711-1-AP

ARL13BA Polyclonal antibody

ARL13BA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The developmental basis of fingerprint pattern formation and variation

Guided self-organization and cortical plate formation in human brain organoids.

Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.

Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium.

A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy.

Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.