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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-17711

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 555-conjugated ARL13B Polyclonal an (CL555-17711)

    IF Staining of MDCK using CL555-17711

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 555 ARL13B antibody (CL555-17711) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-17711 targets ARL13B in IF/ICC applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12015 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
GenBank accession numberBC094725
Gene symbol ARL13B
Gene ID (NCBI) 200894
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
IF protocol for CL Plus 555 ARL13B antibody CL555-17711Download protocol
Standard Protocols
Click here to view our Standard Protocols