Validation Data Gallery

Filter: AllWBIPIHCIF/ICCIF

WB analysis using 17711-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17711-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17711-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 17711-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17711-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 17711-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 17711-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of L02 using 17711-1-AP
IP result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.
IHC staining of mouse brain using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 17711-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 40x lens).
IF Staining of hTERT-RPE1 using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of MDCK using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ARL13B antibody (17711-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of NIH3T3 cells using 17711-1-AP
IF result of anti-ARL13B (17711-1-AP) in NIH3T3 cell by Dr. Sudipto.
IF Staining of hTERT-RPE1 using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).
IF Staining of MDCK using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of hTERT-RPE1 using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).
IF Staining of MDCK using 17711-1-AP
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MDCK using 17711-1-AP
Immunofluorescent images of MDCK cells stained with ARL13B rabbit pAb (17711-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure-Goat anti-Rabbit IgG(H+L) for 17711-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 66200-1-Ig.
IF Staining of hTERT-RPE1 using 17711-1-AP
IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.
IF Staining of mouse embryonic fibroblasts using 17711-1-AP
IF result from Dr. Corbit, Kevin. anti-ARL13B (17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

Featured Product
KD/KO Validated

ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17711-1-AP

Publications(810)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (810)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	L02 cells, NIH/3T3 cells, mouse liver tissue, mouse kidney tissue, mouse brain tissue, rat liver tissue, rat kidney tissue
Positive IP detected in	L02 cells
Positive IHC detected in	mouse brain tissue, human kidney tissue, human liver tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	hTERT-RPE1 cells, NIH3T3 cells, MDCK cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 13 publications below
WB	See 87 publications below
IHC	See 38 publications below
IF	See 725 publications below
IP	See 2 publications below
FC	See 2 publications below

Product Information

17711-1-AP targets ARL13B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARL13B fusion protein Ag12015 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylation factor-like 13B
Calculated molecular weight	48 kDa
Observed molecular weight	40-48 kDa, 60 kDa
GenBank accession number	BC094725
Gene Symbol	ARL13B
Gene ID (NCBI)	200894
RRID	AB_2060867
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q3SXY8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two speciﬁc bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
WB protocol for ARL13B antibody 17711-1-AP	Download protocol
IHC protocol for ARL13B antibody 17711-1-AP	Download protocol
IF protocol for ARL13B antibody 17711-1-AP	Download protocol
IP protocol for ARL13B antibody 17711-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell The developmental basis of fingerprint pattern formation and variation Authors - James D Glover View Article
human	IF	Nat Biotechnol Guided self-organization and cortical plate formation in human brain organoids. Authors - Madeline A Lancaster View Article
mouse	IF	Nature Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche. Authors - David Willnow View Article
mouse	IF	Science Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium. Authors - Avishag Mytlis View Article
human	IF	Nature A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy. Authors - Adriana Sánchez-Danés View Article
mouse	IF	Nature Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Authors - Matthew B Johnson View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17711-1-AP

WB analysis of mouse liver using 17711-1-AP

WB analysis of mouse brain using 17711-1-AP

WB analysis of NIH/3T3 using 17711-1-AP

WB analysis of mouse kidney using 17711-1-AP

WB analysis of L02 using 17711-1-AP

WB analysis using 17711-1-AP

IP experiment of L02 using 17711-1-AP

IHC staining of mouse brain using 17711-1-AP

IHC staining of mouse brain using 17711-1-AP

IHC staining of human testis using 17711-1-AP

IHC staining of human testis using 17711-1-AP

IHC staining of human kidney using 17711-1-AP

IHC staining of human kidney using 17711-1-AP

IHC staining of human liver using 17711-1-AP

IHC staining of human liver using 17711-1-AP

IF Staining of hTERT-RPE1 using 17711-1-AP

IF Staining of MDCK using 17711-1-AP

IF Staining of NIH3T3 cells using 17711-1-AP

IF Staining of hTERT-RPE1 using 17711-1-AP

IF Staining of MDCK using 17711-1-AP

IF Staining of hTERT-RPE1 using 17711-1-AP

IF Staining of MDCK using 17711-1-AP

IF Staining of MDCK using 17711-1-AP

IF Staining of hTERT-RPE1 using 17711-1-AP

IF Staining of mouse embryonic fibroblasts using 17711-1-AP

ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The developmental basis of fingerprint pattern formation and variation

Guided self-organization and cortical plate formation in human brain organoids.

Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.

Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium.

A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy.

Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.