Validation Data Gallery
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Tested Applications
| Positive WB detected in | MKN-45 cells, Calu-3 cells, MDA-MB-231 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60413-1-Ig targets EVI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34143 Product name: Recombinant human EVI1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1032-1230 aa of NM_001105077 Sequence: NSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSALDHIRHFTDSLKMRKMEDNQYSEAELSSFSTSHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSTSHSSSNVWHSMARAAAESSAIQSISHV 相同性解析による交差性が予測される生物種 |
| Full Name | ecotropic viral integration site 1 |
| Calculated molecular weight | 138 kDa |
| Observed molecular weight | 140-150 kDa |
| GenBank accession number | NM_001105077 |
| Gene Symbol | EVI1 |
| Gene ID (NCBI) | 2122 |
| RRID | AB_3670256 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q03112 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EVI1 antibody 60413-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |