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Validation Data Gallery

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  • Western Blot (WB) analysis of MKN-45 cells using EVI1 Monoclonal antibody (60413-1-Ig)

    WB analysis of MKN-45 using 60413-1-Ig (same clone as 60413-1-PBS)

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60413-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EVI1 Monoclonal antibody (60413-1-Ig)

    WB analysis using 60413-1-Ig (same clone as 60413-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60413-1-PBS in a different storage buffer formulation.

EVI1 Monoclonal antibody, PBS Only

EVI1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 60413-1-PBS
Clone No.1A12A6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367, AML1 EVI 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60413-1-PBS targets EVI1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag34143

Product name: Recombinant human EVI1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1032-1230 aa of NM_001105077

Sequence: NSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSALDHIRHFTDSLKMRKMEDNQYSEAELSSFSTSHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSTSHSSSNVWHSMARAAAESSAIQSISHV

相同性解析による交差性が予測される生物種
Full Name ecotropic viral integration site 1
Calculated molecular weight 138 kDa
Observed molecular weight140-150 kDa
GenBank accession numberNM_001105077
Gene Symbol EVI1
Gene ID (NCBI) 2122
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ03112
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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