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Mouse IgG2a isotype control Monoclonal antibody, PBS Only

Mouse IgG2a isotype control Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Megathura crenulata

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

11A1B2

Cat no : 66360-2-PBS

Synonyms

Mouse IgG2a, Mouse IgG2a isotype control

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mouse IgG2a isotype control Monoclonal antibody (66360-2-Ig)

    WB analysis using 66360-2-Ig (same clone as 66360-2-PBS)

    Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Mouse IgG2a isotype control Monoclonal antibody (66360-2-Ig)

    FC experiment of HepG2 using 66360-2-Ig (same clone as 66360-2-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Mouse IgG2a isotype control (66360-2-Ig, Clone: 11A1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Cytochrome c Mouse McAb (66264-1-Ig, Clone: 2D8D11) (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66360-2-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66360-2-PBS targets Mouse IgG2a isotype control in WB, FC, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name Mouse IgG2a isotype control
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment.

Background Information

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).