Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 66264-1-Ig
Various cells were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66264-1-Ig
WB result of Cytochrome c antibody (66264-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells.
WB analysis of HEK-293 using 66264-1-Ig
HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 66264-1-Ig
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 66264-1-Ig
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 66264-1-Ig
human heart tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 66264-1-Ig
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 66264-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66264-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66264-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66264-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66264-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 66264-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 66264-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytochrome c antibody (66264-1-Ig, Clone: 2D8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).
FC experiment of HepG2 using 66264-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Cytochrome c (66264-1-Ig, Clone:2D8D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Featured Product
KD/KO Validated

Cytochrome c Monoclonal antibody

Cytochrome c Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66264-1-Ig

Clone No.2D8D11

Publications(117)

Reviews (1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CYCS, CYC, 2D8D11

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration: 150 μL, 1828 ug/ml

20 μL, 1828 ug/ml

150 μL, 1828 ug/ml

$449/ 150 μL

In stock in Rosemont, IL. Order within 84 hours and 44 mins to receive by Tuesday, April 8

Freight/Packing: $40.00

Quantity

- +

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Buy two full size antibodies and get a third full size primary antibody for free. Use promo code B2G125.

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Overview

Tested Applications
Product Information
Publications (117)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, human heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, HEK-293 cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, RAW 264.7 cells
Positive IHC detected in	human liver cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:1000-1:5000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 106 publications below
IHC	See 2 publications below
IF	See 15 publications below

Product Information

66264-1-Ig targets Cytochrome c in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, canine
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	Cytochrome c fusion protein Ag24349 Predict reactive species
Full Name	cytochrome c, somatic
Calculated Molecular Weight	12 kDa
Observed Molecular Weight	12-15 kDa
GenBank Accession Number	BC009578
Gene Symbol	Cytochrome c
Gene ID (NCBI)	54205
RRID	AB_2716798
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P99999
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Protocols

Product Specific Protocols
WB protocol for Cytochrome c antibody 66264-1-Ig	Download protocol
IHC protocol for Cytochrome c antibody 66264-1-Ig	Download protocol
IF protocol for Cytochrome c antibody 66264-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Hepatology Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation. Authors - Chao Zhang View Article
human	IF	Nat Commun Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. Authors - Wenjun Wang View Article
human	WB	Redox Biol Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma. Authors - Zhongren Xu View Article
human	IF	J. Pineal Res. Human transporters, PEPT1/2, facilitate melatonin transportation into mitochondria of cancer cells: an implication of the therapeutic potential. Authors - Xiaokui Huo View Article
human,mouse	WB	Sci China Life Sci Melatonin decreases GSDME mediated mesothelial cell pyroptosis and prevents peritoneal fibrosis and ultrafiltration failure Authors - Hongxia Ruan View Article
mouse	WB	Br J Pharmacol Substitution of SERCA2 Cys674 accelerates aortic aneurysm by inducing endoplasmic reticulum stress and promoting cell apoptosis. Authors - Langtao Wang View Article