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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cytochrome c Monoclonal antibody (66264-1-Ig)

    WB analysis using 66264-1-Ig (same clone as 66264-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Cytochrome c Monoclonal antibody (66264-1-Ig)

    WB analysis of HepG2 using 66264-1-Ig (same clone as 66264-1-PBS)

    WB result of Cytochrome c antibody (66264-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Cytochrome c Monoclonal antibody (66264-1-Ig)

    WB analysis of HEK-293 using 66264-1-Ig (same clone as 66264-1-PBS)

    HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    WB analysis of rat skeletal muscle using 66264-1-Ig (same clone as 66264-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    WB analysis of mouse skeletal muscle using 66264-1-Ig (same clone as 66264-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    WB analysis of human heart using 66264-1-Ig (same clone as 66264-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    WB analysis of human skeletal muscle using 66264-1-Ig (same clone as 66264-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    IHC staining of human liver cancer using 66264-1-Ig (same clone as 66264-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    IHC staining of human breast cancer using 66264-1-Ig (same clone as 66264-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    IHC staining of human breast cancer using 66264-1-Ig (same clone as 66264-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    IHC staining of human breast cancer using 66264-1-Ig (same clone as 66264-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    IHC staining of human breast cancer using 66264-1-Ig (same clone as 66264-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Cytochrome c Monoclonal antibody (66264-1-Ig)

    IHC staining of human liver cancer using 66264-1-Ig (same clone as 66264-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Cytochrome c Monoclonal antibody (66264-1-Ig)

    IF Staining of HepG2 using 66264-1-Ig (same clone as 66264-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytochrome c antibody (66264-1-Ig, Clone: 2D8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Cytochrome c Monoclonal antibody (66264-1-Ig)

    FC experiment of HepG2 using 66264-1-Ig (same clone as 66264-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Cytochrome c (66264-1-Ig, Clone:2D8D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Cytochrome c Monoclonal antibody, PBS Only

Cytochrome c Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66264-1-PBS
Clone No.2D8D11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CYCS, CYC, 2D8D11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66264-1-PBS targets Cytochrome c in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cytochrome c fusion protein Ag24349 相同性解析による交差性が予測される生物種
Full Name cytochrome c, somatic
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC009578
Gene Symbol Cytochrome c
Gene ID (NCBI) 54205
RRIDAB_2716798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP99999
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.