CoraLite®594-conjugated Cytochrome c Monoclonal antibody
Cytochrome c Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2D8D11
Cat no : CL594-66264
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66264 targets Cytochrome c in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cytochrome c fusion protein Ag24349 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome c, somatic |
| Calculated molecular weight | 12 kDa |
| GenBank accession number | BC009578 |
| Gene symbol | CYCS |
| Gene ID (NCBI) | 54205 |
| RRID | AB_2883547 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 Cytochrome c antibody CL594-66264 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
