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Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of Ramos using 66672-1-Ig (same clone as 66672-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66672-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of human testis using 66672-1-Ig (same clone as 66672-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66672-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of Ramos using 66672-1-Ig (same clone as 66672-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66672-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of Raji using 66672-1-Ig (same clone as 66672-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66672-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of Daudi using 66672-1-Ig (same clone as 66672-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66672-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of HeLa using 66672-1-Ig (same clone as 66672-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66672-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of THP-1 using 66672-1-Ig (same clone as 66672-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66672-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50366-1

    Cytometric bead array standard curve of MP50366-1

    Cytometric bead array standard curve of MP50366-1, PLZF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66672-2-PBS. Detection antibody: 66672-1-PBS. Standard:Ag25180. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50366-2

    Cytometric bead array standard curve of MP50366-2

    Cytometric bead array standard curve of MP50366-2, PLZF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66672-3-PBS. Detection antibody: 66672-1-PBS. Standard:Ag25180. Range: 0.781-100 ng/mL.

PLZF Monoclonal antibody, PBS Only (Detector)

PLZF Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 66672-1-PBS
Clone No.2E6F12

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

ZBTB16, 2E6F12, Zinc finger and BTB domain-containing protein 16, Zinc finger protein 145, Zinc finger protein PLZF

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66672-1-PBS targets PLZF as part of a matched antibody pair:

MP50366-1: 66672-2-PBS capture and 66672-1-PBS detection (validated in Cytometric bead array)

MP50366-2: 66672-3-PBS capture and 66672-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25180

Product name: Recombinant human PLZF protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 101-400 aa of BC026902

Sequence: DLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESR

相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 16
Calculated molecular weight 673 aa, 74 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC026902
Gene Symbol PLZF
Gene ID (NCBI) 7704
RRIDAB_2882026
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ05516
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PLZF, also named as Zinc finger protein 145, is a 673 amino acid protein, which belongs to the krueppel C2H2-type zinc-finger protein family. PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. PLZF may play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues.

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