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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HOXB6 Recombinant antibody (83155-4-RR)

    IF Staining of HepG2 using 83155-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOXB6 antibody (83155-4-RR, Clone: 230433F10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using HOXB6 Recombinant antibody (83155-4-RR)

    IF Staining of A431 using 83155-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using HOXB6 antibody (83155-4-RR, Clone: 230433F10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of K562 using HOXB6 Recombinant antibody (83155-4-RR)

    FC experiment of K562 using 83155-4-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug HOXB6 Recombinant antibody (83155-4-RR, Clone:230433F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83155-4-RR

    Affinity and Kinetic Characterization of 83155-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83155-4-RR against Human HOXB6 were performed. The affinity constant is 0.186 nM.

HOXB6 Recombinant monoclonal antibody

HOXB6 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83155-4-RR
Clone No.230433F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Hox 2.2, Homeobox protein Hu-2, Homeobox protein Hox-B6, Homeobox protein Hox-2B, Homeobox protein Hox-2.2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells, A431 cells
Positive FC (Intra) detected inK562

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83155-4-RR targets HOXB6 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag14189

Product name: Recombinant human HOXB6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-160 aa of BC014651

Sequence: MSSYFVNSTFPVTLASGQESFLGQLPLYSSGYADPLRHYPAPYGPGPGQDKGFATSSYYPPAGGGYGRAAPCDYGPAPAFYREKESACALSGADEQPPFHPEPRKSDCAQDKSVFGETEEQKCSTPVYPWMQRMNSCNSSSFGPSGRRGRQTYTRYQTLE

相同性解析による交差性が予測される生物種
Full Name homeobox B6
Calculated molecular weight 224 aa, 25 kDa
GenBank accession numberBC014651
Gene Symbol HOXB6
Gene ID (NCBI) 3216
RRIDAB_3670849
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP17509
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOXB6 is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development, including that of lung and skin, and has been localized to both the nucleus and cytoplasm. Altered expression of this gene or a change in the subcellular localization of its protein is associated with some cases of acute myeloid leukemia and colorectal cancer.

Protocols

Product Specific Protocols
IF protocol for HOXB6 antibody 83155-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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