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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HOXB6 Recombinant antibody

HOXB6 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230433F10

Cat no : CL488-83155-4

Synonyms

Hox 2.2, Homeobox protein Hu-2, Homeobox protein Hox-B6, Homeobox protein Hox-2B, Homeobox protein Hox-2.2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HOXB6 Recombinant an (CL488-83155-4)

    IF Staining of HepG2 using CL488-83155-4

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HOXB6 antibody (CL488-83155-4, Clone: 230433F10 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated HOXB6 Recombinant an (CL488-83155-4)

    FC experiment of K-562 using CL488-83155-4

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated HOXB6 Recombinant antibody (CL488-83155-4, Clone:230433F10)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83155-4 is HOXB6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HOXB6 fusion protein Ag14189 相同性解析による交差性が予測される生物種
Full Name homeobox B6
Calculated molecular weight 224 aa, 25 kDa
GenBank accession numberBC014651
Gene symbol HOXB6
Gene ID (NCBI) 3216
RRIDAB_3673205
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HOXB6 antibody CL488-83155-4Download protocol
Standard Protocols
Click here to view our Standard Protocols