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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HOXB6 Recombinant antibody

HOXB6 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230433F10

Cat no : 83155-4-RR

Synonyms

Hox 2.2, Homeobox protein Hu-2, Homeobox protein Hox-B6, Homeobox protein Hox-2B, Homeobox protein Hox-2.2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HOXB6 Recombinant antibody (83155-4-RR)

    IF Staining of HepG2 using 83155-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOXB6 antibody (83155-4-RR, Clone: 230433F10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using HOXB6 Recombinant antibody (83155-4-RR)

    IF Staining of A431 using 83155-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using HOXB6 antibody (83155-4-RR, Clone: 230433F10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of K562 using HOXB6 Recombinant antibody (83155-4-RR)

    FC experiment of K562 using 83155-4-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug HOXB6 Recombinant antibody (83155-4-RR, Clone:230433F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83155-4-RR

    Affinity and Kinetic Characterization of 83155-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83155-4-RR against Human HOXB6 were performed. The affinity constant is 0.186 nM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells, A431 cells
Positive FC (Intra) detected inK562

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83155-4-RR is HOXB6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HOXB6 fusion protein Ag14189 相同性解析による交差性が予測される生物種
Full Name homeobox B6
Calculated molecular weight 224 aa, 25 kDa
GenBank accession numberBC014651
Gene symbol HOXB6
Gene ID (NCBI) 3216
RRIDAB_3670849
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOXB6 is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development, including that of lung and skin, and has been localized to both the nucleus and cytoplasm. Altered expression of this gene or a change in the subcellular localization of its protein is associated with some cases of acute myeloid leukemia and colorectal cancer.

Protocols

Product Specific Protocols
IF protocol for HOXB6 antibody 83155-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols