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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated 14-3-3 Sigma Monoclonal antibody

14-3-3 Sigma Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F10D9

Cat no : CL594-66251

Synonyms

14 3 3 protein sigma, 14-3-3 Sigma, HME1, SFN, stratifin, YWHAS

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated 14-3-3 Sigma Monoclonal an (CL594-66251)

    IF Staining of MCF-7 using CL594-66251

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®594 14-3-3 Sigma antibody (CL594-66251, Clone: 3F10D9 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated 14-3-3 Sigma Monoclonal an (CL594-66251)

    FC experiment of A431 using CL594-66251

    1X10^6 A431 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human 14-3-3 Sigma (CL594-66251, Clone:3F10D9) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66251 targets 14-3-3 Sigma in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen 14-3-3 Sigma fusion protein Ag12644 相同性解析による交差性が予測される生物種
Full Name stratifin
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC002995
Gene symbol 14-3-3 Sigma
Gene ID (NCBI) 2810
RRIDAB_2919970
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

Protocols

Product Specific Protocols
IF protocol for CL594 14-3-3 Sigma antibody CL594-66251Download protocol
FC protocol for CL594 14-3-3 Sigma antibody CL594-66251Download protocol
Standard Protocols
Click here to view our Standard Protocols