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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal (67666-1-Ig)

    WB analysis using 67666-1-Ig

    E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration.

  • ELISA experiment of Recombinant protein using SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal (67666-1-Ig)

    ELISA experiment of Recombinant protein using 67666-1-Ig

    Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

  • ELISA experiment of Recombinant protein using SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal (67666-1-Ig)

    ELISA experiment of Recombinant protein using 67666-1-Ig

    Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

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SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal antibody

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal Antibody for WB, ELISA
Cat No. 67666-1-Ig
Clone No.1B3C3
Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

Virus and More (1)

Applications

WB, ELISA

Synonyms

2019-nCOV nucleocapsid phosphoprotein, COVID-19 N protein, N protein, nucleocapsid phosphoprotein, SARS-COV-2 N protein, SARS-COV-2 nucleocapsid phosphoprotein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inAg30676
Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67666-1-Ig targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676 相同性解析による交差性が予測される生物種
Full Name COVID-19 N Protein
GenBank accession numberNC_045512
Gene Symbol COVID-19 N Protein
Gene ID (NCBI) 43740575
RRIDAB_2882862
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.

Protocols

Product Specific Protocols
WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 67666-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Biosens Bioelectron

Electronic field effect detection of SARS-CoV-2 N-protein before the onset of symptoms.

Authors - I Novodchuk
View Article

Viruses

Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins.

Authors - Marina Pribanić Matešić
View Article

J Virol

Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation

Authors - Zhaohuan Wang
View Article