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Tested Applications
| Positive WB detected in | Ag30676 |
| Positive ELISA detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67666-1-Ig targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with Virus samples.
| Tested Reactivity | Virus |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676 相同性解析による交差性が予測される生物種 |
| Full Name | COVID-19 N Protein |
| GenBank accession number | NC_045512 |
| Gene Symbol | COVID-19 N Protein |
| Gene ID (NCBI) | 43740575 |
| RRID | AB_2882862 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 67666-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biosens Bioelectron Electronic field effect detection of SARS-CoV-2 N-protein before the onset of symptoms. | ||
J Virol Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation |