Virus SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal antibody, PBS Only (Capture)

E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

Sandwich ELISA standard curve of MP50061-1, Virus 2019-nCOV nucleocapsid phosphoprotein Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67666-1-PBS. Detection antibody: HRP-conjugated 67666-2-PBS. Standard: Ag30676. Range: 0.5-20 ng/mL.

Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.