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  • Featured Product
  • KD/KO Validated

4-1BBL/TNFSF9 Monoclonal antibody, PBS Only (Capture)

4-1BBL/TNFSF9 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4B1

Cat no : 66450-1-PBS

Synonyms

TNFSF9, CD137L, 4-1BB ligand, 4 1BBL, 4 1BB ligand

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using 4-1BBL/TNFSF9 Monoclonal antibody (66450-1-Ig)

    WB analysis of Raji using 66450-1-Ig (same clone as 66450-1-PBS)

    Raji, Ramos, Daudi, and K-562 cells were subjected to SDS PAGE followed by western blot with 66450-1-Ig (TNFSF9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66450-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50011-1

    Sandwich ELISA standard curve of MP50011-1

    Sandwich ELISA standard curve of MP50011-1, 4-1BBL/TNFSF9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66450-1-PBS. Detection antibody: HRP-conjugated 66450-2-PBS. Standard: Ag12768. Range: 0.313-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66450-1-PBS targets 4-1BBL/TNFSF9 as part of a matched antibody pair:

MP50011-1: 66450-1-PBS capture and 66450-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen 4-1BBL/TNFSF9 fusion protein Ag12768 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 9
Calculated molecular weight 254 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC104805
Gene symbol TNFSF9
Gene ID (NCBI) 8744
RRIDAB_2881819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.