- Featured Product
- KD/KO Validated
4EBP1 Monoclonal antibody, PBS Only
4EBP1 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, rat, pig, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2C3F3
Cat no : 60246-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
60246-1-PBS targets 4EBP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, pig, mouse samples.
Tested Reactivity | human, rat, pig, mouse |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | 4EBP1 fusion protein Ag18985 相同性解析による交差性が予測される生物種 |
Full Name | eukaryotic translation initiation factor 4E binding protein 1 |
Calculated molecular weight | 118 aa, 12 kDa |
Observed molecular weight | 15-24 kDa |
GenBank accession number | BC004459 |
Gene symbol | EIF4EBP1 |
Gene ID (NCBI) | 1978 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS only |
Storage Conditions | Store at -80°C. |
Background Information
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)