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Validation Data Gallery

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  • Western Blot (WB) analysis of K562 using 4EBP1 Monoclonal antibody (60246-1-Ig)

    WB analysis of K562 using 60246-1-Ig (same clone as 60246-1-PBS)

    WB result of 4EBP1 antibody (60246-1-Ig, 1:4000) with si-Control and si-4EBP1 transfected K562 cells. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using 4EBP1 Monoclonal antibody (60246-1-Ig)

    WB analysis of K-562 using 60246-1-Ig (same clone as 60246-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCI-H1299 cells using 4EBP1 Monoclonal antibody (60246-1-Ig)

    WB analysis of NCI-H1299 using 60246-1-Ig (same clone as 60246-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HCT 116 cells using 4EBP1 Monoclonal antibody (60246-1-Ig)

    WB analysis of HCT 116 using 60246-1-Ig (same clone as 60246-1-PBS)

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using 4EBP1 Monoclonal antibody (60246-1-Ig)

    WB analysis of K-562 using 60246-1-Ig (same clone as 60246-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using 4EBP1 Monoclonal antibody (60246-1-Ig)

    IHC staining of human pancreas cancer using 60246-1-Ig (same clone as 60246-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using 4EBP1 Monoclonal antibody (60246-1-Ig)

    IHC staining of human colon cancer using 60246-1-Ig (same clone as 60246-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using 4EBP1 Monoclonal antibody (60246-1-Ig)

    IHC staining of human colon cancer using 60246-1-Ig (same clone as 60246-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using 4EBP1 Monoclonal antibody (60246-1-Ig)

    IF Staining of HeLa using 60246-1-Ig (same clone as 60246-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60246-1-Ig(4EBP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

4EBP1 Monoclonal antibody, PBS Only

4EBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60246-1-PBS
Clone No.2C3F3

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60246-1-PBS targets 4EBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen 4EBP1 fusion protein Ag18985 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4E binding protein 1
Calculated molecular weight 118 aa, 12 kDa
Observed molecular weight 15-24 kDa
GenBank accession numberBC004459
Gene Symbol EIF4EBP1
Gene ID (NCBI) 1978
RRIDAB_2881368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13541
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)