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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AADACL1 Polyclonal antibody, PBS Only

AADACL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14021-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using AADACL1 Polyclonal antibody (14021-1-AP)

    WB analysis using 14021-1-AP (same clone as 14021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14021-1-AP (AADACL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using AADACL1 Polyclonal antibody (14021-1-AP)

    IP experiment of COLO 320 using 14021-1-AP (same clone as 14021-1-PBS)

    IP result of anti-AADACL1 (IP:14021-1-AP, 4ug; Detection:14021-1-AP 1:500) with COLO 320 cells lysate 1320 ug. This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human brain using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human brain using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human spleen using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human spleen using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human lung using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human lung using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human ovary using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human kidney using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human kidney using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human ovary using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human skin using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using AADACL1 Polyclonal antibody (14021-1-AP)

    IHC staining of human skin using 14021-1-AP (same clone as 14021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using AADACL1 Polyclonal antibody (14021-1-AP)

    IF Staining of COLO 320 using 14021-1-AP (same clone as 14021-1-PBS)

    Immunofluorescent analysis of COLO 320 cells using 14021-1-AP (AADACL1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14021-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14021-1-PBS targets AADACL1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AADACL1 fusion protein Ag5136 相同性解析による交差性が予測される生物種
Full Name arylacetamide deacetylase-like 1
Calculated molecular weight 46 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC047588
Gene symbol AADACL1
Gene ID (NCBI) 57552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AADACL1(Arylacetamide deacetylase-like 1) is also named as NCEH1, KIAA1363 and belongs to the 'GDXG' lipolytic enzyme family. The transmembrane enzyme, AADACL1, controls the production of the monoalkylglycerol ether (MAGE) class of NELs in cancer cells and acts as a 2-acetyl MAGE hydrolase and is likely the principal source for this activity in tumor cells(PMID:21513884). The full length protein has three glycosylation sites and can be N-glycosylated(PMID:19159218). It has 3 isoforms produced by alternative splicing.