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AATF Monoclonal antibody

AATF Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2A6G6

Cat no : 67868-1-Ig

Synonyms

Protein AATF, HSPC277, DED, CHE1, CHE 1


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Tested Applications

Positive WB detected inHCT 116 cells, Neuro-2a cells, HepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67868-1-Ig targets AATF in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AATF fusion protein Ag0195 相同性解析による交差性が予測される生物種
Full Name apoptosis antagonizing transcription factor
Calculated molecular weight 63 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC000591
Gene symbol AATF
Gene ID (NCBI) 26574
RRIDAB_2918626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AATF antibody 67868-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Onco Targets Ther

AATF is Overexpressed in Human Head and Neck Squamous Cell Carcinoma and Regulates STAT3/Survivin Signaling

Authors - Lin Fu
humanWB,IHC

Onco Targets Ther

AATF is Overexpressed in Human Bladder Cancer and Regulates Chemo-Sensitivity Through Survivin.

Authors - Shutao Tan