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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AATF Monoclonal antibody

AATF Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A6G6

Cat no : CL488-67868

Synonyms

Protein AATF, HSPC277, DED, CHE1, CHE 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated AATF Monoclonal anti (CL488-67868)

    FC experiment of HeLa using CL488-67868

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AATF (CL488-67868, Clone:2A6G6) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67868 targets AATF in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AATF fusion protein Ag0195 相同性解析による交差性が予測される生物種
Full Name apoptosis antagonizing transcription factor
Calculated molecular weight 63 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC000591
Gene symbol AATF
Gene ID (NCBI) 26574
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apoptosis antagonizing transcription factor (AATF), an interacting partner of RNA polymerase II is a multifunctional protein that is highly conserved in eukaryotes. In addition to nuclear hormone receptors, AATF has been shown to interact with several transcription factors, including the retinoblastoma protein (pRb), p65, and STAT3(PMID: 34785906). Furthermore AATF serves as a critical regulator in various cancers and promotes tumorigenesis by protecting cancer cells from apoptosis induction, favoring cell proliferation, or promoting cell survival by autophagy(PMID: 33145763).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 AATF antibody CL488-67868Download protocol
Standard Protocols
Click here to view our Standard Protocols