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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCE1 Monoclonal antibody (67960-1-Ig)

    WB analysis using 67960-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of various lysates using ABCE1 Monoclonal antibody (67960-1-Ig)

    WB analysis using 67960-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ABCE1 Monoclonal antibody (67960-1-Ig)

    IHC staining of human breast cancer using 67960-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67960-1-Ig (ABCE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ABCE1 Monoclonal antibody (67960-1-Ig)

    IF Staining of U2OS using 67960-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ABCE1 Monoclonal antibody (67960-1-Ig)

    IF Staining of U2OS using 67960-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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ABCE1 Monoclonal antibody

ABCE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67960-1-Ig
Clone No.1B7E8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, A431 cells, U2OS cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67960-1-Ig targets ABCE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ABCE1 fusion protein Ag28493 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family E (OABP), member 1
Calculated molecular weight 599 aa, 67 kDa
Observed molecular weight68 kDa
GenBank accession numberBC016283
Gene Symbol ABCE1
Gene ID (NCBI) 6059
RRIDAB_2918711
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61221
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.

Protocols

Product Specific Protocols
WB protocol for ABCE1 antibody 67960-1-IgDownload protocol
IHC protocol for ABCE1 antibody 67960-1-IgDownload protocol
IF protocol for ABCE1 antibody 67960-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols