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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABCE1 Monoclonal antibody, PBS Only

ABCE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7E8

Cat no : 67960-1-PBS

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCE1 Monoclonal antibody (67960-1-Ig)

    WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ABCE1 Monoclonal antibody (67960-1-Ig)

    WB analysis using 67960-1-Ig (same clone as 67960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ABCE1 Monoclonal antibody (67960-1-Ig)

    IHC staining of human breast cancer using 67960-1-Ig (same clone as 67960-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67960-1-Ig (ABCE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ABCE1 Monoclonal antibody (67960-1-Ig)

    IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ABCE1 Monoclonal antibody (67960-1-Ig)

    IF Staining of U2OS using 67960-1-Ig (same clone as 67960-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67960-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67960-1-PBS targets ABCE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ABCE1 fusion protein Ag28493 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family E (OABP), member 1
Calculated molecular weight 599 aa, 67 kDa
Observed molecular weight68 kDa
GenBank accession numberBC016283
Gene symbol ABCE1
Gene ID (NCBI) 6059
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.