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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABL1 Monoclonal antibody

ABL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E7A5

Cat no : 68254-1-Ig

Synonyms

c ABL, JTK7, EC:2.7.10.2, ABL, Abelson tyrosine-protein kinase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABL1 Monoclonal antibody (68254-1-Ig)

    WB analysis using 68254-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68254-1-Ig (ABL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ABL1 Monoclonal antibody (68254-1-Ig)

    IF Staining of HepG2 using 68254-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ABL1 Monoclonal antibody (68254-1-Ig)

    IF Staining of U2OS using 68254-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ABL1 Monoclonal antibody (68254-1-Ig)

    IF Staining of MCF-7 using 68254-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of K-562 cells using ABL1 Monoclonal antibody (68254-1-Ig)

    FC experiment of K-562 using 68254-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ABL1 (68254-1-Ig, Clone:1E7A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inU2OS cells, HepG2 cells, MCF-7 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68254-1-Ig targets ABL1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ABL1 fusion protein Ag26152 相同性解析による交差性が予測される生物種
Full Name c-abl oncogene 1, receptor tyrosine kinase
Calculated molecular weight 123 kDa
Observed molecular weight 140 kDa
GenBank accession numberNM_005157
Gene symbol ABL1
Gene ID (NCBI) 25
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABL1, also named as ABL, JTK7, c-ABL and p150, belongs to the protein kinase superfamily, Tyr protein kinase family and ABL subfamily. ABL1 regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. ABL1 localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. It regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. ABL1 catalyze the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The antibody is specific to ABL1.

Protocols

Product Specific Protocols
WB protocol for ABL1 antibody 68254-1-IgDownload protocol
IF protocol for ABL1 antibody 68254-1-IgDownload protocol
FC protocol for ABL1 antibody 68254-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols