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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ACAT1 Polyclonal antibody

ACAT1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16215

Synonyms

MAT, EC:2.3.1.9, Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, Acetoacetyl CoA thiolase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ACAT1 Polyclonal ant (CL488-16215)

    IF Staining of HeLa using CL488-16215

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ACAT1 antibody (CL488-16215) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated ACAT1 Polyclonal ant (CL488-16215)

    FC experiment of HeLa using CL488-16215

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ACAT1 (CL488-16215) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16215 targets ACAT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAT1 fusion protein Ag9215 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A acetyltransferase 1
Calculated molecular weight 45 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC010942
Gene symbol ACAT1
Gene ID (NCBI) 38
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ACAT1 antibody CL488-16215Download protocol
FC protocol for CL Plus 488 ACAT1 antibody CL488-16215Download protocol
Standard Protocols
Click here to view our Standard Protocols