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ACAT2 Monoclonal antibody

ACAT2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F5F11

Cat no : 68005-1-Ig

Synonyms

ACAT2, ACTL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACAT2 Monoclonal antibody (68005-1-Ig)

    WB analysis using 68005-1-Ig

    Various cell lysates were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using ACAT2 Monoclonal antibody (68005-1-Ig)

    WB analysis of HepG2 using 68005-1-Ig

    WB result of ACAT2 antibody (68005-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells.

  • Western Blot (WB) analysis of HCT 116 cells using ACAT2 Monoclonal antibody (68005-1-Ig)

    WB analysis of HCT 116 using 68005-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ACAT2 Monoclonal antibody (68005-1-Ig)

    WB analysis using 68005-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ACAT2 Monoclonal antibody (68005-1-Ig)

    IHC staining of human placenta using 68005-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68005-1-Ig (ACAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACAT2 Monoclonal antibody (68005-1-Ig)

    IF Staining of HepG2 using 68005-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (68005-1-Ig, Clone: 1F5F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HCT 116 cells, rat brain tissue, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue, rabbit brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68005-1-Ig targets ACAT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACAT2 fusion protein Ag6596 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A acetyltransferase 2
Calculated molecular weight 41 kDa
Observed molecular weight42 kDa
GenBank accession numberBC000408
Gene symbol ACAT2
Gene ID (NCBI) 39
RRIDAB_2918752
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACAT2 antibody 68005-1-IgDownload protocol
IHC protocol for ACAT2 antibody 68005-1-IgDownload protocol
IF protocol for ACAT2 antibody 68005-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Phytomedicine

Investigating the potential mechanism and therapeutic effects of SLXG for cholesterol gallstone treatment

Authors - Yang Wang
View Article