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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ACAT2 Monoclonal antibody

ACAT2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F5F11

Cat no : CL488-68005

Synonyms

ACAT2, acetoacetyl CoA thiolase, Acetyl-CoA acetyltransferase, cytosolic, Acetyl-CoA transferase-like protein, ACTL, Cytosolic acetoacetyl-CoA thiolase, EC:2.3.1.9, 1F5F11

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ACAT2 Monoclonal ant (CL488-68005)

    IF Staining of HepG2 using CL488-68005

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ACAT2 antibody (CL488-68005, Clone: 1F5F11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated ACAT2 Monoclonal ant (CL488-68005)

    FC experiment of HepG2 using CL488-68005

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ACAT2 (CL488-68005, Clone:1F5F11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68005 targets ACAT2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACAT2 fusion protein Ag6596 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A acetyltransferase 2
Calculated molecular weight 41 kDa
Observed molecular weight42 kDa
GenBank accession numberBC000408
Gene symbol ACAT2
Gene ID (NCBI) 39
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ACAT2 antibody CL488-68005Download protocol
FC protocol for CL Plus 488 ACAT2 antibody CL488-68005Download protocol
Standard Protocols
Click here to view our Standard Protocols