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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated ACLY Monoclonal antibody

ACLY Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E10D5

Cat no : CL594-67166

Synonyms

ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®594-conjugated ACLY Monoclonal antibody (CL594-67166)

    IF Staining of A549 using CL594-67166

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 ACLY antibody (CL594-67166, Clone: 1E10D5 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67166 targets ACLY in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen ACLY fusion protein Ag7709 相同性解析による交差性が予測される生物種
Full Name ATP citrate lyase
Calculated molecular weight 121 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC006195
Gene symbol ACLY
Gene ID (NCBI) 47
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 ACLY antibody CL594-67166Download protocol
Standard Protocols
Click here to view our Standard Protocols