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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACPP Monoclonal antibody, PBS Only

ACPP Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G2C9

Cat no : 60176-1-PBS

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using ACPP Monoclonal antibody (60176-1-Ig)

    WB analysis of K-562 using 60176-1-Ig (same clone as 60176-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using ACPP Monoclonal antibody (60176-1-Ig)

    WB analysis of human testis using 60176-1-Ig (same clone as 60176-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using ACPP Monoclonal antibody (60176-1-Ig)

    IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60176-1-PBS targets ACPP in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ACPP fusion protein Ag8578 相同性解析による交差性が予測される生物種
Full Name acid phosphatase, prostate
Calculated molecular weight 418 aa, 48 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC007460
Gene symbol ACPP
Gene ID (NCBI) 55
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).