ACPP Monoclonal antibody
ACPP Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1G2C9
Cat no : 60176-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, human testis tissue |
Positive IHC detected in | human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human prostate cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
60176-1-Ig targets ACPP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | ACPP fusion protein Ag8578 相同性解析による交差性が予測される生物種 |
Full Name | acid phosphatase, prostate |
Calculated molecular weight | 418 aa, 48 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC007460 |
Gene symbol | ACPP |
Gene ID (NCBI) | 55 |
RRID | AB_10733336 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ACPP antibody 60176-1-Ig | Download protocol |
IHC protocol for ACPP antibody 60176-1-Ig | Download protocol |
IF protocol for ACPP antibody 60176-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Endocrinol (Lausanne) Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans. |