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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ACPP Monoclonal antibody

ACPP Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G2C9

Cat no : CL488-60176

Synonyms

PAP, ACP 3, acid phosphatase, prostate, Acid phosphatase 3, 5'-nucleotidase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using CoraLite® Plus 488-conjugated ACPP Monoclonal anti (CL488-60176)

    IF Staining of human prostate cancer using CL488-60176

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 ACPP antibody (CL488-60176, Clone: 1G2C9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ACPP Monoclonal anti (CL488-60176)

    IF Staining of HeLa using CL488-60176

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ACPP antibody (CL488-60176, Clone: 1G2C9 ) at dilution of 1:200, CL594-phalloidin (red).

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Tested Applications

Positive IF-P detected inhuman prostate cancer tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60176 targets ACPP in IF/ICC, IF-P applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ACPP fusion protein Ag8578 相同性解析による交差性が予測される生物種
Full Name acid phosphatase, prostate
Calculated molecular weight 418 aa, 48 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC007460
Gene symbol ACPP
Gene ID (NCBI) 55
RRIDAB_2919233
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ACPP antibody CL488-60176Download protocol
Standard Protocols
Click here to view our Standard Protocols