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Alpha Actinin Polyclonal antibody

Name: Alpha Actinin Polyclonal antibody
Brand: Proteintech
SKU: 11313-2-AP
Rating: 5.00 (2 reviews)

Alpha Actinin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 11313-2-AP

Synonyms

Alpha actinin 1, ACTN1, Alpha-actinin cytoskeletal isoform, ACTN 1, actinin, alpha 1

Validation Data Gallery

Filter:

AllWBIHCIF-PFC (Intra)

WB analysis using 11313-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11313-2-AP (Alpha Actinin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11313-2-AP
WB result of Alpha Actinin antibody (11313-2-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Actinin transfected HeLa cells.
WB analysis of mouse spleen using 11313-2-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 11313-2-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 11313-2-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 11313-2-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 10x lens).
IHC staining of human colon using 11313-2-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11313-2-AP (Alpha Actinin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat heart using 11313-2-AP
Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 11313-2-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11313-2-AP (alpha Actinin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66219-1-Ig (N-cadherin) in red.
IF Staining of mouse skeletal muscle using 11313-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse skeletal muscle using 11313-2-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 11313-2-AP
Immunofluorescent analysis of (-20°C Methanol) fixed mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of rat heart using 11313-2-AP
Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of A431 using 11313-2-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (11313-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 11313-2-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	NIH/3T3 cells, HeLa cells, mouse spleen tissue, rat heart tissue, C6 cells
Positive IHC detected in	human heart tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	rat heart tissue, mouse heart tissue, mouse skeletal muscle tissue
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 21 publications below
IHC	See 1 publications below
IF	See 11 publications below

Product Information

11313-2-AP targets Alpha Actinin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Alpha Actinin fusion protein Ag1859 相同性解析による交差性が予測される生物種
Full Name	actinin, alpha 1
Calculated molecular weight	103 kDa
Observed molecular weight	100-105 kDa
GenBank accession number	BC015766
Gene symbol	Alpha Actinin
Gene ID (NCBI)	87
RRID	AB_2223815
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
WB protocol for Alpha Actinin antibody 11313-2-AP	Download protocol
IHC protocol for Alpha Actinin antibody 11313-2-AP	Download protocol
IF protocol for Alpha Actinin antibody 11313-2-AP	Download protocol
FC protocol for Alpha Actinin antibody 11313-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Redox Biol MK-886 protects against cardiac ischaemia/reperfusion injury by activating proteasome-Keap1-NRF2 signalling Authors - Kai-Na Shi View Article
human	WB	J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK Authors - Li Cui View Article
human	IF	Adv Healthc Mater A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction. Authors - Zhi Zheng View Article
mouse	WB	Cell Death Differ Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration. Authors - Ming Zou View Article
mouse	IF	Transl Res Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling. Authors - Pengyu Jia View Article
mouse	WB	Theranostics miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury. Authors - Jing Shi View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Jason (Verified Customer) (10-29-2019)	Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue. Applications: Immunohistochemistry, Primary Antibody Dilution: 1/100 Cell Tissue Type: Mouse myocardium Alpha Actinin Antibody Immunohistochemistry, validation (1/100 dilution) in Mouse myocardium (Cat no:11313-2-AP)
FH Steeve (Verified Customer) (01-08-2018)	Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin Applications: Western Blot Primary Antibody Dilution: 1:1'1000 Cell Tissue Type: human umbilical veins

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha Actinin Polyclonal antibody

Alpha Actinin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 11313-2-AP

WB analysis of HeLa using 11313-2-AP

WB analysis of mouse spleen using 11313-2-AP

WB analysis of rat heart using 11313-2-AP

IHC staining of human heart using 11313-2-AP

IHC staining of human heart using 11313-2-AP

IHC staining of human colon using 11313-2-AP

IF Staining of rat heart using 11313-2-AP

IF Staining of mouse heart using 11313-2-AP

IF Staining of mouse skeletal muscle using 11313-2-AP

IF Staining of mouse skeletal muscle using 11313-2-AP

IF Staining of mouse heart using 11313-2-AP

IF Staining of rat heart using 11313-2-AP

FC experiment of A431 using 11313-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MK-886 protects against cardiac ischaemia/reperfusion injury by activating proteasome-Keap1-NRF2 signalling

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.

Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Reviews