Validation Data Gallery
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Tested Applications
| Positive IF-P detected in | rat heart tissue |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-11313 targets Alpha Actinin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Alpha Actinin fusion protein Ag1859 相同性解析による交差性が予測される生物種 |
| Full Name | actinin, alpha 1 |
| Calculated molecular weight | 103 kDa |
| Observed molecular weight | 100-105 kDa |
| GenBank accession number | BC015766 |
| Gene Symbol | Alpha Actinin |
| Gene ID (NCBI) | 87 |
| RRID | AB_3083899 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12814 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Alpha Actinin antibody CL488-11313 | Download protocol |
| FC protocol for CL Plus 488 Alpha Actinin antibody CL488-11313 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |