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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Alpha Actinin Polyclonal antibody

Alpha Actinin Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11313

Synonyms

Alpha actinin 1, ACTN1, Alpha-actinin cytoskeletal isoform, ACTN 1, actinin, alpha 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using CoraLite® Plus 488-conjugated Alpha Actinin Polycl (CL488-11313)

    IF Staining of rat heart using CL488-11313

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using CoraLite® Plus 488-conjugated Alpha Actinin Polycl (CL488-11313)

    IF Staining of rat heart using CL488-11313

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated Alpha Actinin Polycl (CL488-11313)

    FC experiment of A431 using CL488-11313

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Alpha Actinin (CL488-11313) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inrat heart tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11313 targets Alpha Actinin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actinin fusion protein Ag1859 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 1
Calculated molecular weight 103 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC015766
Gene symbol Alpha Actinin
Gene ID (NCBI) 87
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Alpha Actinin antibody CL488-11313Download protocol
FC protocol for CL Plus 488 Alpha Actinin antibody CL488-11313Download protocol
Standard Protocols
Click here to view our Standard Protocols