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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACTN3 Recombinant antibody, PBS Only

ACTN3 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241168A5

Cat no : 84356-1-PBS

Synonyms

Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3, actinin, alpha 3

Validation Data Gallery

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  • Western Blot (WB) analysis of rat skeletal muscle tissue using ACTN3 Recombinant antibody (84356-1-RR)

    WB analysis of rat skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84356-1-RR (ACTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ACTN3 Recombinant antibody (84356-1-RR)

    IHC staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84356-1-RR (ACTN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using ACTN3 Recombinant antibody (84356-1-RR)

    IF Staining of mouse skeletal muscle using 84356-1-RR (same clone as 84356-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (84356-1-RR, Clone: 241168A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84356-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84356-1-RR

    Affinity and Kinetic Characterization of 84356-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84356-1-RR against Human ACTN3 were performed. The affinity constant is 0.116 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84356-1-PBS is ACTN3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACTN3 fusion protein Ag36709 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 3
Calculated molecular weight 901 aa, 103 kDa
Observed molecular weight100 kDa
GenBank accession numberBC099647
Gene symbol ACTN3
Gene ID (NCBI) 89
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.