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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACTN3 Recombinant antibody

ACTN3 Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241168A5

Cat no : 84356-1-RR

Synonyms

Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3, actinin, alpha 3

Validation Data Gallery

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  • Western Blot (WB) analysis of rat skeletal muscle tissue using ACTN3 Recombinant antibody (84356-1-RR)

    WB analysis of rat skeletal muscle using 84356-1-RR

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84356-1-RR (ACTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ACTN3 Recombinant antibody (84356-1-RR)

    IHC staining of mouse skeletal muscle using 84356-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84356-1-RR (ACTN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using ACTN3 Recombinant antibody (84356-1-RR)

    IF Staining of mouse skeletal muscle using 84356-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (84356-1-RR, Clone: 241168A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84356-1-RR

    Affinity and Kinetic Characterization of 84356-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84356-1-RR against Human ACTN3 were performed. The affinity constant is 0.116 nM.

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Tested Applications

Positive WB detected inrat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84356-1-RR is ACTN3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACTN3 fusion protein Ag36709 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 3
Calculated molecular weight 901 aa, 103 kDa
Observed molecular weight100 kDa
GenBank accession numberBC099647
Gene symbol ACTN3
Gene ID (NCBI) 89
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACTN3 antibody 84356-1-RRDownload protocol
IHC protocol for ACTN3 antibody 84356-1-RRDownload protocol
IF protocol for ACTN3 antibody 84356-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols