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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ACTN3 Polyclonal antibody

ACTN3 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24378

Synonyms

F-actin cross-linking protein, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using CoraLite® Plus 488-conjugated ACTN3 Polyclonal ant (CL488-24378)

    IF Staining of mouse skeletal muscle using CL488-24378

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CoraLite® Plus 488 ACTN3 antibody (CL488-24378) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of C2C12 cells using CoraLite® Plus 488-conjugated ACTN3 Polyclonal ant (CL488-24378)

    FC experiment of C2C12 using CL488-24378

    1x10^6 C2C12 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Actn3 Polyclonal Antibody (CL488-24378)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse skeletal muscle tissue
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24378 targets ACTN3 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTN3 fusion protein Ag19479 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 3
Calculated molecular weight 901 aa, 103 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC099647
Gene symbol ACTN3
Gene ID (NCBI) 89
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ACTN3 antibody CL488-24378Download protocol
FC protocol for CL Plus 488 ACTN3 antibody CL488-24378Download protocol
Standard Protocols
Click here to view our Standard Protocols