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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using ACVRL1 Monoclonal antibody (66857-1-Ig)

    WB analysis of HUVEC using 66857-1-Ig (same clone as 66857-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of JAR cells using ACVRL1 Monoclonal antibody (66857-1-Ig)

    WB analysis of JAR using 66857-1-Ig (same clone as 66857-1-PBS)

    JAR cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using ACVRL1 Monoclonal antibody (66857-1-Ig)

    WB analysis of human placenta using 66857-1-Ig (same clone as 66857-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ACVRL1 Monoclonal antibody (66857-1-Ig)

    WB analysis of mouse brain using 66857-1-Ig (same clone as 66857-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using ACVRL1 Monoclonal antibody (66857-1-Ig)

    WB analysis of Daudi using 66857-1-Ig (same clone as 66857-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ACVRL1 Monoclonal antibody (66857-1-Ig)

    WB analysis of Jurkat using 66857-1-Ig (same clone as 66857-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ACVRL1 Monoclonal antibody (66857-1-Ig)

    IHC staining of human lymphoma using 66857-1-Ig (same clone as 66857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66857-1-Ig (ACVRL1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ACVRL1 Monoclonal antibody (66857-1-Ig)

    IHC staining of human lymphoma using 66857-1-Ig (same clone as 66857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66857-1-Ig (ACVRL1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66857-1-PBS in a different storage buffer formulation.

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ACVRL1 Monoclonal antibody, PBS Only

ACVRL1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66857-1-PBS
Clone No.2E11B10

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Activin receptor like kinase 1, ACVRL1, ACVRLK1, ALK 1, ALK1, HHT, HHT2, ORW2, SKR3, TSR I

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66857-1-PBS targets ACVRL1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACVRL1 fusion protein Ag6446 相同性解析による交差性が予測される生物種
Full Name activin A receptor type II-like 1
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa, 63 kDa
GenBank accession numberBC042637
Gene Symbol ACVRL1
Gene ID (NCBI) 94
RRIDAB_2882196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP37023
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.