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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADAM10 Monoclonal antibody

ADAM10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A6B7

Cat no : 66620-1-Ig

Synonyms

AD10, ADAM 10, ADAM10, CD156c, CDw156, HsT18717, kuz, Kuzbanian protein homolog, MADM

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using ADAM10 Monoclonal antibody (66620-1-Ig)

    WB analysis of THP-1 using 66620-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADAM10 Monoclonal antibody (66620-1-Ig)

    WB analysis using 66620-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ADAM10 Monoclonal antibody (66620-1-Ig)

    IHC staining of human prostate cancer using 66620-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ADAM10 Monoclonal antibody (66620-1-Ig)

    IHC staining of human prostate cancer using 66620-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inTHP-1 cells, MCF-7 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66620-1-Ig targets ADAM10 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADAM10 fusion protein Ag23062 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 10
Calculated molecular weight 748 aa, 84 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberBC126253
Gene symbol ADAM10
Gene ID (NCBI) 102
RRIDAB_2881980
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).

Protocols

Product Specific Protocols
WB protocol for ADAM10 antibody 66620-1-IgDownload protocol
IHC protocol for ADAM10 antibody 66620-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Arch Biochem Biophys

Maresin1 restrains chronic inflammation and Aβ production to ameliorate Alzheimer's disease via modulating ADAM10/17 and its associated neuroprotective signal pathways: A pilot study

Authors - Shuang Sun
  • KD Validated
View Article