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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADAM10 Monoclonal antibody, PBS Only

ADAM10 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A6B7

Cat no : 66620-1-PBS

Synonyms

AD10, ADAM 10, ADAM10, CD156c, CDw156, HsT18717, kuz, Kuzbanian protein homolog, MADM

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using ADAM10 Monoclonal antibody (66620-1-Ig)

    WB analysis of THP-1 using 66620-1-Ig (same clone as 66620-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ADAM10 Monoclonal antibody (66620-1-Ig)

    WB analysis using 66620-1-Ig (same clone as 66620-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ADAM10 Monoclonal antibody (66620-1-Ig)

    IHC staining of human prostate cancer using 66620-1-Ig (same clone as 66620-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ADAM10 Monoclonal antibody (66620-1-Ig)

    IHC staining of human prostate cancer using 66620-1-Ig (same clone as 66620-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66620-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66620-1-PBS targets ADAM10 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADAM10 fusion protein Ag23062 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 10
Calculated molecular weight 748 aa, 84 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberBC126253
Gene symbol ADAM10
Gene ID (NCBI) 102
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).