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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADAM17 Monoclonal antibody

ADAM17 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F4C2

Cat no : 68725-1-Ig

Synonyms

ADAM 17, ADAM17, CD156b, cSVP, Snake venom like protease, TACE, TNF alpha convertase, TNF alpha converting enzyme

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADAM17 Monoclonal antibody (68725-1-Ig)

    WB analysis using 68725-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADAM17 Monoclonal antibody (68725-1-Ig)

    WB analysis using 68725-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inU2OS cells, NIH/3T3 cells, Calu-3 cells, A549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68725-1-Ig targets ADAM17 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADAM17 fusion protein Ag34022 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 17
Calculated molecular weight 824 aa, 93 kDa
Observed molecular weight 90-110 kDa
GenBank accession numberBC136783
Gene symbol ADAM17
Gene ID (NCBI) 6868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

Protocols

Product Specific Protocols
WB protocol for ADAM17 antibody 68725-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols