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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADAM17 Monoclonal antibody, PBS Only (Detector)

ADAM17 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F4C2

Cat no : 68725-1-PBS

Synonyms

Snake venom-like protease, EC:3.4.24.86, Disintegrin and metalloproteinase domain-containing protein 17, cSVP, ADAM 17

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ADAM17 Monoclonal antibody (68725-1-Ig)

    WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ADAM17 Monoclonal antibody (68725-1-Ig)

    WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68725-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50392-2

    Cytometric bead array standard curve of MP50392-2

    Cytometric bead array standard curve of MP50392-2, ADAM17 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68725-2-PBS. Detection antibody: 68725-1-PBS. Standard:Ag34022. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68725-1-PBS targets ADAM17 as part of a matched antibody pair:

MP50392-2: 68725-2-PBS capture and 68725-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADAM17 fusion protein Ag34022 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 17
Calculated molecular weight 824 aa, 93 kDa
Observed molecular weight 90-110 kDa
GenBank accession numberBC136783
Gene symbol ADAM17
Gene ID (NCBI) 6868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).