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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADAM17 Recombinant antibody (84292-4-RR)

    WB analysis using 84292-4-RR (same clone as 84292-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84292-4-RR (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01169-3

    Sandwich ELISA standard curve of MP01169-3

    Sandwich ELISA standard curve of MP01169-3, Human ADAM17 Recombinant Matched Antibody Pair - PBS only. 84292-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32418. 84292-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Sample test of MP01169-3

    Sample test of MP01169-3

    The mean ADAM17 concentration was determined to be 18.51 ng/mL in THP-1 cell extract based on a 4.4 mg/mL extract load and 13.76 ng/mL in A549 cell extract based on a 2.7 mg/mL extract load.

  • Sample test of MP01169-3

    Sample test of MP01169-3

    Serum of six individual healthy human donors was measured. The human ADAM17 concentration of detected samples was determined to be 1,898.9 pg/mL with a range of 21.5 - 8,096.2 ng/mL.

  • Flow cytometry (FC) experiment of HCT 116 cells using ADAM17 Recombinant antibody (84292-4-RR)

    FC experiment of HCT 116 using 84292-4-RR (same clone as 84292-4-PBS)

    1x10^6 HCT 116 cells were intracellularly stained with 0.25 ug ADAM17 Recombinant antibody (84292-4-RR, Clone:241305A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84292-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84292-4-RR

    Affinity and Kinetic Characterization of 84292-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84292-4-RR against Human ADAM17 were performed. The affinity constant is 0.385 nM.

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ADAM17 Recombinant antibody, PBS Only (Detector)

ADAM17 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84292-4-PBS
Clone No.241305A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84292-4-PBS targets ADAM17 as part of a matched antibody pair:

MP01169-3: 84292-3-PBS capture and 84292-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ADAM17 fusion protein Ag32418 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 17
Calculated molecular weight 824 aa, 93 kDa
GenBank accession numberBC136783
Gene Symbol ADAM17
Gene ID (NCBI) 6868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP78536
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).