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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADAM17 Recombinant antibody (84292-4-RR)

    WB analysis using 84292-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84292-4-RR (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84292-4-RR

    Affinity and Kinetic Characterization of 84292-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84292-4-RR against Human ADAM17 were performed. The affinity constant is 0.385 nM.

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ADAM17 Recombinant antibody

ADAM17 Recombinant Antibody for WB, ELISA
Cat No. 84292-4-RR
Clone No.241305A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Snake venom-like protease, EC:3.4.24.86, Disintegrin and metalloproteinase domain-containing protein 17, cSVP, ADAM 17

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HCT 116 cells, HUVEC cells, SW480 cells, HeLa cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84292-4-RR targets ADAM17 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ADAM17 fusion protein Ag32418 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase domain 17
Calculated molecular weight 824 aa, 93 kDa
GenBank accession numberBC136783
Gene Symbol ADAM17
Gene ID (NCBI) 6868
RRIDAB_3671836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP78536
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

Protocols

Product Specific Protocols
WB protocol for ADAM17 antibody 84292-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols