Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse placenta tissue, NIH/3T3 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 10 publications below |
| IF | See 1 publications below |
Product Information
24934-1-AP targets ADAMTS12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ADAMTS12 fusion protein Ag20712 相同性解析による交差性が予測される生物種 |
| Full Name | ADAM metallopeptidase with thrombospondin type 1 motif, 12 |
| Calculated molecular weight | 178 kDa |
| Observed molecular weight | 155 kDa |
| GenBank accession number | BC058841 |
| Gene Symbol | ADAMTS12 |
| Gene ID (NCBI) | 81792 |
| RRID | AB_2879806 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P58397 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAMTS12 antibody 24934-1-AP | Download protocol |
| IF protocol for ADAMTS12 antibody 24934-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells. | ||
Hum Mol Genet Epigenetic changes in reterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth.
| ||
Cell Signal ADAMTS12 promotes oxaliplatin chemoresistance and angiogenesis in gastric cancer through VEGF upregulation | ||
Sci Rep ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer.
| ||
Int J Mol Med Overexpression of PRDM13 inhibits glioma cells via Rho and GTP enzyme activation protein. | ||
Hepatobiliary Pancreat Dis Int ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer.
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