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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite® Plus 488-conjugated ADAMTS12 Polyclonal (CL488-24934)

    IF Staining of NIH/3T3 using CL488-24934

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 ADAMTS12 antibody (CL488-24934) at dilution of 1:200.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated ADAMTS12 Polyclonal antibody

ADAMTS12 Polyclonal Antibody for IF/ICC
Cat No. CL488-24934

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

ADAM-TS12, ADAM-TS 12, ADAMTS 12, ADAM TS12, ADAM TS 12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24934 targets ADAMTS12 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAMTS12 fusion protein Ag20712 相同性解析による交差性が予測される生物種
Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 12
Calculated molecular weight 178 kDa
Observed molecular weight 155 kDa
GenBank accession numberBC058841
Gene Symbol ADAMTS12
Gene ID (NCBI) 81792
RRIDAB_3672782
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58397
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ADAMTS12 antibody CL488-24934Download protocol
Standard Protocols
Click here to view our Standard Protocols