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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of HEK-293T using 67884-1-Ig

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis using 67884-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis using 67884-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of mouse brain using 67884-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of HeLa using 67884-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of mouse cerebellum using 67884-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of mouse brain using 67884-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ADC Monoclonal antibody (67884-1-Ig)

    IF Staining of A431 using 67884-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ADC antibody (67884-1-Ig, Clone: 1A2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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ADC Monoclonal antibody

ADC Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67884-1-Ig
Clone No.1A2B11
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

ADC, Antizyme inhibitor 2, ARGDC, arginine decarboxylase, AZI2, AZIN2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue, HEK-293 cells, pig brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67884-1-Ig targets ADC in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADC fusion protein Ag31049 相同性解析による交差性が予測される生物種
Full Name arginine decarboxylase
Calculated molecular weight 460 aa, 50 kDa
Observed molecular weight48-50 kDa
GenBank accession numberBC028128
Gene Symbol ADC
Gene ID (NCBI) 113451
RRIDAB_2918641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96A70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.

Protocols

Product Specific Protocols
WB protocol for ADC antibody 67884-1-IgDownload protocol
IF protocol for ADC antibody 67884-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Lighting up arginine metabolism reveals its functional diversity in physiology and pathology

Authors - Rui Li
View Article