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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADC Monoclonal antibody, PBS Only

ADC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2B11

Cat no : 67884-1-PBS

Synonyms

ADC, Antizyme inhibitor 2, ARGDC, arginine decarboxylase, AZI2, AZIN2, KIAA1945, ODC p, ODC paralogue, ODC1L, ODCP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis using 67884-1-Ig (same clone as 67884-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis using 67884-1-Ig (same clone as 67884-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of HEK-293T using 67884-1-Ig (same clone as 67884-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of HeLa using 67884-1-Ig (same clone as 67884-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of mouse cerebellum using 67884-1-Ig (same clone as 67884-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of mouse brain using 67884-1-Ig (same clone as 67884-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ADC Monoclonal antibody (67884-1-Ig)

    WB analysis of mouse brain using 67884-1-Ig (same clone as 67884-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67884-1-Ig (ADC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ADC Monoclonal antibody (67884-1-Ig)

    IF Staining of A431 using 67884-1-Ig (same clone as 67884-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ADC antibody (67884-1-Ig, Clone: 1A2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67884-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67884-1-PBS targets ADC in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADC fusion protein Ag31049 相同性解析による交差性が予測される生物種
Full Name arginine decarboxylase
Calculated molecular weight 460 aa, 50 kDa
Observed molecular weight48-50 kDa
GenBank accession numberBC028128
Gene symbol ADC
Gene ID (NCBI) 113451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.