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CoraLite® Plus 488-conjugated ADH1B Monoclonal antibody
ADH1B Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F1B4
Cat no : CL488-66939
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66939 targets ADH1B in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ADH1B fusion protein Ag10630 相同性解析による交差性が予測される生物種 |
| Full Name | alcohol dehydrogenase 1B (class I), beta polypeptide |
| Calculated molecular weight | 375 aa, 40 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC033009 |
| Gene symbol | ADH1B |
| Gene ID (NCBI) | 125 |
| RRID | AB_2919392 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ADH1B antibody CL488-66939 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
