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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADH4 Monoclonal antibody (67303-1-Ig)

    WB analysis using 67303-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67303-1-Ig (ADH4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ADH4 Monoclonal antibody (67303-1-Ig)

    IF Staining of HEK-293 using 67303-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ADH4 antibody (67303-1-Ig, Clone: 3D12E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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ADH4 Monoclonal antibody

ADH4 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67303-1-Ig
Clone No.3D12E9

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

ADH 2, ADH4, Alcohol dehydrogenase 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, HuH-7 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67303-1-Ig targets ADH4 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADH4 fusion protein Ag9884 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 4 (class II), pi polypeptide
Calculated molecular weight 380 aa, 40 kDa
Observed molecular weight 40-43 kDa
GenBank accession numberBC022319
Gene Symbol ADH4
Gene ID (NCBI) 127
RRIDAB_2882565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08319
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ADH4 antibody 67303-1-IgDownload protocol
IF protocol for ADH4 antibody 67303-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols